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Inhibition of TrxR1 activity by C55 in vitro . A) rat liver TrxR1 and B) 10 µg of protein samples extracted from human HCC <t>(HepG2,</t> Huh7) or BCa (MCF-7, MDA-MB-231) cells were incubated with the indicated concentrations of C55 and then TrxR1 activity was assayed as described in material and methods. Kit inhibitor was used as positive control inhibitor for TrxR1. Values are expressed as percentage of activity compared to solvent alone ± SD of at least two separate experiments.
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Inhibition of TrxR1 activity by C55 in vitro . A) rat liver TrxR1 and B) 10 µg of protein samples extracted from human HCC (HepG2, Huh7) or BCa (MCF-7, MDA-MB-231) cells were incubated with the indicated concentrations of C55 and then TrxR1 activity was assayed as described in material and methods. Kit inhibitor was used as positive control inhibitor for TrxR1. Values are expressed as percentage of activity compared to solvent alone ± SD of at least two separate experiments.

Journal: Journal of Enzyme Inhibition and Medicinal Chemistry

Article Title: Structure-based identification of a non-covalent thioredoxin reductase inhibitor with proven ADMET suitability

doi: 10.1080/14756366.2025.2585606

Figure Lengend Snippet: Inhibition of TrxR1 activity by C55 in vitro . A) rat liver TrxR1 and B) 10 µg of protein samples extracted from human HCC (HepG2, Huh7) or BCa (MCF-7, MDA-MB-231) cells were incubated with the indicated concentrations of C55 and then TrxR1 activity was assayed as described in material and methods. Kit inhibitor was used as positive control inhibitor for TrxR1. Values are expressed as percentage of activity compared to solvent alone ± SD of at least two separate experiments.

Article Snippet: Human HCC HepG2 (HB-8065 TM ) cells were obtained from the American Type Culture Collection (ATCC, Rockville, MD).

Techniques: Inhibition, Activity Assay, In Vitro, Incubation, Positive Control, Solvent

Effects of C55 treatment on cell viability in HCC (HepG2, Huh7) and BCa (MCF-7, MDA-MB-231) cell lines. Cells were treated with different concentrations of C55 for 72 (A) or 96 (B) hours. Cell viability was determined by using MTS assay.

Journal: Journal of Enzyme Inhibition and Medicinal Chemistry

Article Title: Structure-based identification of a non-covalent thioredoxin reductase inhibitor with proven ADMET suitability

doi: 10.1080/14756366.2025.2585606

Figure Lengend Snippet: Effects of C55 treatment on cell viability in HCC (HepG2, Huh7) and BCa (MCF-7, MDA-MB-231) cell lines. Cells were treated with different concentrations of C55 for 72 (A) or 96 (B) hours. Cell viability was determined by using MTS assay.

Article Snippet: Human HCC HepG2 (HB-8065 TM ) cells were obtained from the American Type Culture Collection (ATCC, Rockville, MD).

Techniques: MTS Assay

Effects of C55 on oxidative stress signalling. A) Gene expression analysis by real-time PCR in HepG2 and MDA-MB-231 cells treated for 48 h with the indicated concentrations of C55 and Auranofin (AUR). * p < 0.05, ** p < 0.01, C55 or AUR versus control vehicle alone B) ROS generation in HepG2 and MDA-MB-231 cells treated with C55. Cells were untreated or treated with C55 or AUR at the indicated concentrations for 48 h, and intracellular ROS levels were evaluated using H 2 DCFDA as a probe. Data are expressed as the percentage of control cells and are the means ± SD of two separate experiments, each performed in triplicate. * p < 0.05, C55 or AUR versus control vehicle alone. C) Western blotting analysis of γ-H2AX in HepG2 and MDA-MB-231 cells treated for 48 h with the indicated concentrations of C55 and AUR.

Journal: Journal of Enzyme Inhibition and Medicinal Chemistry

Article Title: Structure-based identification of a non-covalent thioredoxin reductase inhibitor with proven ADMET suitability

doi: 10.1080/14756366.2025.2585606

Figure Lengend Snippet: Effects of C55 on oxidative stress signalling. A) Gene expression analysis by real-time PCR in HepG2 and MDA-MB-231 cells treated for 48 h with the indicated concentrations of C55 and Auranofin (AUR). * p < 0.05, ** p < 0.01, C55 or AUR versus control vehicle alone B) ROS generation in HepG2 and MDA-MB-231 cells treated with C55. Cells were untreated or treated with C55 or AUR at the indicated concentrations for 48 h, and intracellular ROS levels were evaluated using H 2 DCFDA as a probe. Data are expressed as the percentage of control cells and are the means ± SD of two separate experiments, each performed in triplicate. * p < 0.05, C55 or AUR versus control vehicle alone. C) Western blotting analysis of γ-H2AX in HepG2 and MDA-MB-231 cells treated for 48 h with the indicated concentrations of C55 and AUR.

Article Snippet: Human HCC HepG2 (HB-8065 TM ) cells were obtained from the American Type Culture Collection (ATCC, Rockville, MD).

Techniques: Gene Expression, Real-time Polymerase Chain Reaction, Control, Western Blot